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A series of hybrid country-focused meetings took place between NARES, national and regional stakeholders, CGIAR breeders and F2R-CWANA WP2 researchers to promote the fast-track release of the most suitable Genetic Innovations for the CWANA region. Participants were familiarized with OneCGIAR strategies to disseminate Genetic Innovations and were asked to prioritize, firstly, the crops and crop- Target Product Profiles (TPPs) available in the OneCGIAR portfolio that were considered most important for the countries. Prioritization was based, among other considerations, on their socioeconomic importance for the country and the current availability of suitable varieties targeting the TPPs selected. Secondly, newly released but underutilized varieties that best fit the selected TPPs were also prioritized. The prioritized county and crop-level target product profiles and newly released varieties will be the basis of subsequent activities related to developing and releasing genetic innovations within CWANA under WP2. This record is for the stakeholder engagement meeting in Morocco.

الزراعة بالمقحات البرية (فاب) في المناطق شبه الجافة - فلسطين

Conservation Agriculture is a sustainable agricultural production system guided by three core principles: no (or minimal) tillage to the soil after harvest; permanent soil cover to lock in moisture and reduce evaporation; and crop diversification replacing monocropping - to enhance soil health and subsequently improve crop productivity and household resilience.

The growing demand for native planting material in ecological restoration and rehabilitation for agro-silvo-pastoral ecosystems has resulted in a major global industry in their sourcing, multiplication, and sale. Plant tissue culture is used for producing high-quality, disease-free, and true-to-type plants at a fast rate. Micropropagation can help to meet the increasing demand for planting material and afforestation programs. However, in vitro plant propagation is an expensive technique compared to conventional methods using suckers, seeds, and cuttings. Therefore, adopting measures to lower production costs without compromising plant quality is essential. This can be achieved by improving the culture media composition. Incorporating organic growth additives can stimulate tissue growth and increase the number of shoots, leaves, and roots in culture media. Organic growth supplementation speeds up the formation and development of cultures and yields vigorous plants. Plant regeneration from meristems (shoot tips and axillary buds) is a reliable way to produce true-to-type plants compared with callus and somatic embryogenesis regeneration, but in vitro culture environments can be mutagenic. Therefore, detecting somaclonal variations at an early stage of development is considered crucial in propagating plants. The genetic stability of in vitro regenerated plants needs to be ascertained by using DNA-based molecular markers. This review aims to provide up-to-date research progress on incorporating organic growth additives to enhance in vitro tissue culture protocols and to emphasize the importance of using PCR-based molecular markers such as RAPD, ISSR, SSR, and SCoT. The review was assessed based on the peer-reviewed works published in scientific databases including Science Direct, Scopus, Springer, JSTOR, onlinelibrary, and Google Scholar.

The presence of pollinating insects in crop fields is an essential factor for agricultural production and pollinator conservation. Agricultural intensification has been identified as a driver of pollinator decline over the last decades and challenges the efficiency of pollination. Several approaches are used to support pollinators and their ecosystem services, notably reward-based wildflower strips. ‘Farming with Alternative Pollinators’ (FAP) aims to attract and sustain pollinators using marketable habitat enhancement plants (MHEP) in the field borders instead of wildflowers. These MHEP are selected in conjunction with farmers. We tested here whether the FAP approach increases diversity and abundance of flower visitors in melon fields in a semi-arid landscape in Morocco. Moreover, we examined whether MHEP increase flower-visitor abundance in melon flowers. We recorded a total of 1330 insect specimens including 573 specimens of wild bees. Lasioglossum malachurum was the major flower visitor in melon and several MHEP. As flower-visitor abundance and diversity in FAP fields were higher than in control fields, we conclude that FAP can be a valuable approach for pollinator protection in agro-ecosystems; 16.5% of wild bees and wasps showed spillover from the field borders to the melon fields.

In Morocco, the intensive use of agricultural land coupled with irregular precipitation is a serious threat to the country’s food security. Conservation agriculture especially no-tillage (NT) system has shown an important result in the semi-arid regions of Morocco, but its dissemination to other, more humid, agro-ecological zones (precipita tion > 350 mm) is still low. For this purpose, a field experiment under NT system has been installed since 2004 in the Zaer Plateau (Central Morocco) to study the adaptation of this system to the irregular rainfall compared to a conventional tillage (CT). Yields (grain and biomass) of crops (wheat and lentil) under NT and CT were analyzed and compared over the years of study. The ANOVA test showed that yields over the seven years were significantly different and that both crop yields under NT system were greater than or equal to those under CT system even though lentil is more vulnerable to extreme climate events under CT and NT systems. Unlike NT, yields under CT were significantly correlated with the rainfall amount received during the crop cycle. This indicates the dependence of CT precipitation, whereas NT is more adaptable to the irregularities of the climate in the study area.

Barley leaf rust caused by Puccinia hordei (Ph) is one of the major limiting biotic stresses of barley production worldwide and causes yield losses of up to 60%. A diversity panel of 316 barley genotypes (AM2017) composed of released cultivars, advanced breeding lines and landraces was screened for Ph resistance at the seedling stage using two isolates (SRT-SAT and SRT-MRC), while the adult plant stage resistance screening was conducted at the disease hotspot location of Sidi Allal Tazi (SAT) for the cropping seasons of 2017 and 2019. The phenotypic responses were combined with 36,793 single nucleotide polymorphism (SNP) markers in a genome-wide association study (GWAS) using the general linear model (GLM), mixed linear model (MLM), settlement of MLM under progressively exclusive relationship (SUPER), multiple-locus MLM (MLMM), fixed and random model circulating probability unification (FarmCPU), and Bayesian-information and linkage-disequilibrium iteratively nested keyway (BLINK) in GAPIT3, and MLM (K+Q), MLM (K+PCA), and GLM (Q) models in TASSEL to identify genomic regions linked to Ph resistance. Fourteen barley genotypes were resistant (R) at the seedling stage to both Ph isolates, SRT-SAT and SRT-MRC, and twelve genotypes were either resistant (R) or moderately resistant (MR) at the adult plant stage, whereas only one genotype was resistant at the seedling stage, and moderately resistant at the adult plant stage. The genome scan revealed 58 significant marker trait associations (MTA) among which 34 were associated with seedling resistance (SR) and 24 with adult plant resistance (APR). Common genomic regions conferring resistance to Ph were identified at both stages on chromosome 2H (106.53 cM and at 107.37 cM), and on chromosome 7H (126.7 cM). Among the 58 MTA identified, 26 loci had been reported in previous studies, while the remaining 32 loci were regarded as novel. Furthermore, the functional annotation of candidate genes (CGs) adjacent to 36 SNP markers with proteins involved in disease resistance further confirms that some of the SNP markers from our study could be associated with Ph resistance in barley. The resistant barley genotypes and some of the SNP markers from this study with high R2 and additive effects can be converted into high-throughput functional markers for accelerated selection and pyramiding of leaf rust resistance genes in North African barley germplasm.

The aim of this R4D Initiatives is to explore the profitability of manufactured feed pellets for small-scale crop-livestock farmers in Tunisia. During the past few growing seasons, within several R4D initiatives and projects (the SWC@Scale as part of the global GIZ ProSol Program; the CRP-Livestock “feed and forages”, and the International Fund for Agricultural Development (IFAD) funded CLCA phase 2), ICARDA and its national partners have introduced imported feed pellet machines that were donated with a 10% financial contribution (to ensure strong ownership of the technology) to some pre-selected professional farmer organizations (FO) (SMSA and GDA5) and individual farmers including agri-preneurs, with the idea of developing small feed businesses using these machines and boosting the nutrition of local herds. After assessing the available feed resources for each region (Northern, Center, and Southern), the leading members of each FO, under the supervision of the project’s scientific teams, developed various feeding formulas. Other farmers have been developing their own formulas based on consultation with local extension agents. Some of the formulas used by farmers are still under experimentation and will be the subject of this brief, aiming at validating their economic viabilities.